Metal complex separation with on-line sample preconcentration in micellar electrokinetic chromatography.

Development of a Novel On-line Sample Preconcentration and Separation Technique in Microchip Micellar Electrokinetic Chromatography

Twenty-five years of micellar electrokinetic chromatography

Micellar electrokinetic chromatography (MEKC), which can separate neutral analytes as well as charged analytes by the capillary electrophoretic technique, was developed in 1982 and the first paper was published in 1984. The authors’ group concentrated their effort into the characterization of MEKC as a separation technique until early 1990s. Most issues in MEKC separations were successfully sol...

On-line preconcentration and determination of ketamine and norketamine by micellar electrokinetic chromatography. Complementary method to gas chromatography/mass spectrometry.

We have investigated a rapid, simple, and highly efficient on-line preconcentration method using in micellar electrokinetic chromatography (MEKC) for the analysis of abused drugs. Ketamine is an anesthetic that has been abused as a hallucinogen. We applied the sample sweeping technique first to ketamine and its major metabolite, norketamine, and separated the analytes with MEKC. Several of the ...

Fluorescence Imaging Analysis of Transient Trapping–microchip Micellar Electrokinetic Chromatography

This paper reports an fluorescence imaging analysis to verify the proposed mechanisms in transient trapping– microchip micellar electrokinetic chromatography (tr-trapping–MCMEKC). Fluorescence imaging revealed that a process of tr-trapping consisted of four migration stages with different apparent diffusion constants. In the first trapping stage, the diffusion of a focused sample band was suppr...

Improvement of derivatized amino acid detection sensitivity in micellar electrokinetic capillary chromatography by means of acid-induced pH-mediated stacking technique

Derivatization is a frequently used sample preparation procedure applicable to the enhancement of analyte detection sensitivity. Amino acids mostly require derivatization prior to electrophoretic or chromatographic analysis, especially if spectrophotometric detection is used. This study presents an on-line preconcentration technique for derivatized amino acids. The sensitivity of the method was...